Gen Bio 2 -Recombinant DNA

Which enzyme could be used to join two DNA fragments?

• DNA ligase

• DNA polymerase

• DNA recombinase

• DNA endonuclease

Which statement about recombinant DNA is true?

• All restriction endonucleases generate blunt end fragments.

• Hydrogen bonds between base pairs are very strong.

• Restriction endonucleases cut both strands of DNA at the same time.

• Sticky ends prevent DNA ligase activity.

Why are plant cells, as opposed to animal cells, particularly useful as hosts for recombinant DNA?

• Animal cells cannot be cultured.

• Plant cells can make totipotent stem cells.

• Plant cells have reverse transcriptase.

• Plant cells have plasmids

Genetic engineering involves

• cutting out a DNA sequence.

• changing a DNA sequence.

• reinserting DNA into living organisms.

• all of the above

A recombinant plasmid gets inside a bacterial cell by

• . hybridization

• radiation

• transformation

• recombination

A DNA molecule produced by combining DNA from different sources is known as

• a mutant

• a change

• strange DNA

• recombinant DNA

The process of making changes in the DNA code of living organisms is called

• selective breeding

• genetic engineering

• inbreeding

• hybridization

Small circular pieces of DNA found inside bacteria in addition to their chromosome is called

• a DNA

• a clone

• RNA

• a plasmid

What does it mean to “clone a gene”?

• make many copies of the gene

• remove the gene

• change the gene

• destroy the gene

DNA from a human has been inserted into a bacterial plasmid and reinserted back into the bacterium. The bacterium now contains both human DNA and bacterial DNA. The bacterium is now considered a

• mutation

• clone

• PCR

• transgenic organism