Column Chromatography

Mobile phase can be the following, EXCEPT......

• liquid

• gas

• supercritical fluid

• supercritical gas

An open column chromatography system consists of C18-modified silica as the stationary phase and a mixture of acetonitrile-formic acid 0.1% (1:1) as the mobile phase. This system is categorized as....

• normal-phase chromatography

• reverse-phase chromatography

• gradient chromatography

• isocratic chromatography

A mixture of methyl red and methyl orange is eluted from a silica column using chloroform, followed by ethanol. This is a/an ................. elution

• isocratic

• gradient

• perpendicular

• linear

Which of the following solvent has the strongest elution power on a silica column?

• Acetonitrile

• Ethanol

• Hexane

• Ethyl acetate

The following column chromatography systems use pressure to flow the mobile phase, EXCEPT.....

• flash chromatography

• open column chromatography

• high-performance liquid chromatography

• gas chromatography

A column packing method in which stationary phase particles are dispersed in a suitable solvent, then poured into the column is called .......

• dry-pack method

• dispersion method

• slurry method

• gravity method

The x-axis in an open-column chromatogram is ......................

• Retention time

• Migration distance

• Retention volume

• Elution time

Which of the following parameter is affected by peak widths?

• Resolution

• Selectivity

• Retention

• Capacity

To separate proteins from salts, a/an ............................ column can be used.

• affinity

• size-exclusion

• adsorption

• partition

Which of the following is used for analyte quantification in a column chromatography?

• retention volume

• retention time

• peak height

• peak area