Electrophoresis Review

For which of the following would it NOT be appropriate to use electrophoresis as part of analysis?

• Separating proteins from a cell

• DNA fingerprinting

• Separation of enzymes based on their activity

• Paternity test

TRUE OR FALSE?

Agarose gel is used in Electrophoresis of protein.

• True

• False

_________________ gel is a cross-linked polymer of acrylamide. Its concentration determines the length of chains in the gel.

What is the function of sodium dodecyl sulfate in electrophoresis?

• to track the passage of the sample

• a detergent that will denature proteins

• a reducing agent

• to increase the density of the sample

What is the function of glycerol in electrophoresis?

• to track the passage of the sample

• a detergent that will denature proteins

• a reducing agent

• to increase the density of the sample density

The wells in the gel are position next to which of the electrodes for protein electrophoresis?

• negative

• positive

Into which gel are the wells formed ?

• separating gel

• stacking gel

• sample gel

• bottom gel

In in the blank.

The most commonly used staining solution for SDA-PAGE electrophoresis is called __________________________

Which of the following are true for isoelectric focusing?

• proteins separate according to their isoelectric point

• protein stop at the isoelectric point

• DNA are separated according to their isoelectric point

• no further procedure is necessary to separate the proteins

What is ethidium bromide used for during electrophoresis?

• used to denature proteins

• used as a tracking gel

• used to visualised DNA fragments in uv light