Electrophoresis

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

Why do bacteria have restriction enzymes?

• To prevent being infected by viruses
• To help DNA get into the cell
• To be able to grow in an incubator
• They don't - viruses have restriction enzymes

Which two bear species are most closely related?

• Bear 1 and Bear 2
• Bear 1 and Bear 3
• Bear 2 and Bear 3
• There’s no way to tell

Which of these is NOT a use for DNA Fingerprinting?

• Identifying the person whose DNA was left at a crime scene

• Determining the father of a child

• Finding the likelihood of inheriting a genetic disorder

• Figuring out which species are more closely related

Shannon does not know who her father is.  There are three possibilities:  Jack, Daniel and Bill.  Who is the father?

• Jack
• Daniel
• Bill

A lab technician needs more DNA to work with. They can use ___ to make more DNA copies.

• PCR (Polymerase Chain Reaction)

• electrophoresis

• cloning

• genetic engineering