QS5 Installation Training - Mediven Penang

What are the 3 stages of PCR in sequence?

• Denaturation, Extension, Annealing

• Denaturation, Annealing, Extension

• Annealing, Extension, Denaturation

• Annealing, Denaturation, Extension

Multiplexing can be performed using SYBR Green assay

• True

• False

Choose the FALSE statement.

• Both PCR and qPCR requires fluorescence dye

• qPCR does not require gel electrophoresis

• PCR is more time consuming than qPCR

How many VeriFlex zones available in QS5 system?

• 2

• 3

• 4

• 5

• 6

What is the maximum zone-to-zone temperature difference for QS5?

• 5

• 10

• 15

Which statement is TRUE about cycle threshold?

• Threshold level is above the baseline signal

• Cycle threshold is the number of cycle required for fluorescence signal falls below the baseline signal

• The lower the Ct value is, the higher concentration of target nucleic acid is in the sample

• Threshold is set in exponential phase

Choose the FALSE statement.

• Real time PCR requires fluorescence detection

• Real time PCR is also known as RT-PCR

• A PCR run must always have a NTC control

• RNA must be reverse transcribed before PCR

What is the file format of QS5 data file?

• .edt

• .eda

• .edf

• .eds

What is the acceptable signal for determining optimal dye concentration in 96-well plate?

• 400,000

• 3,000,000

• 600,000

• 4,000,000

What is the criteria in successful custom dye calibration?

• Uniform signals with peaks aligned with dye's wavelength

• Highest dye concentration that falls within the acceptable signal range