Which of the following is NOT a way farmers would traditionally modify plants in the past?

• Transgenic manipulation

• Saving seeds

• Selective breeding

• Cross-pollinating

What macromolecule do genes code for?

• Proteins

• Carbohydrates

• Nucleic acids

• Lipids

What is another term for transgenic manipulation?

• Selective breeding

• Genetic engineering

• Cross-pollinating

• Seed saving

A gene that comes from the same organism to make a GMO can best be described as...

(ex: gene from corn placed into corn plant)

• Cisgenic

• Transgenic

Why can Agrobacterium be used to genetically modify a crop?

• Agrobacterium causes disease (attacks!) in plants.

• Agrobacterium has the ability to transfer a portion of its DNA into plant cells.

• The Agrobacterium carries the vector with the gene of interest & helps to incorporate its DNA with the crop's DNA.

• All of the above!

What is transformation?

• The process of cutting DNA with restriction enzymes.

• The process of the gene of interest being put in the vector.

• The process of the gene of interest being taken into the host cell's DNA & being expressed on the outside of the cell.

• The process selecting a gene of interest to cut with restriction enzymes.

Which would NOT be a reason to use GM crops?

• Using GM crops can increase crop yields.

• Using GM crops can reduce use of insecticide sprays.

• Using GM crops may be able to feed more people faster.

• Using GM crops can increase pesticide resistance in pests.

How might scientists check to make sure their GMO worked?

• Add a fluorescent (glowing) gene (with the gene of interest) to see if the final product glows

• Add another gene (with the gene of interest), like a resistance to herbicide gene, to test for its addition in the final product.

• Add pests to the final product to see if they live or die!

• All of the above!

How many steps to produce GMOs?

• 3

• 4

• 5

• 6

Can YOU explain your thoughts on GMOs using evidence and reasoning from your lab activities?

• Yes

• No