Crispr Gene Editing

What does the acronym CRISPR stand for?

• Controlled Reservative Image Spectacle Palindromic Recounts

• Clustered Regularly-Interspaced Short Palindromic Repeats

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria

Why do bacteria have CRISPR? Check all that apply.

• To be able to remember which viruses they have been infected with

• To be able to edit virus DNA

• To cut apart virus DNA thereby stopping the virus infection

• To be able to edit their own DNA

What does the cas9 protein do?

• Prevents viruses from entering bacteria

• Cut RNA

• Cut DNA

• Binds to RNA

We have been modifying DNA sequences for many years - ever since restriction enzymes were discovered in the early 1970's. CRISPR and restriction enzymes are essentially the same because they can both be used to cut DNA and can be used inside of living cells.

• TRUE

• FALSE - Restriction enzymes do not cut DNA

• FALSE - CRISPR does not cut DNA

• FALSE - Restriction enzymes cannot be used inside of living cells

How does CRISPR edit DNA? Check ALL that apply.

• CRISPR chemically changes A's to C's

• CRISPR cuts the DNA and the cell makes errors when it tries to fix the broken DNA

• CRISPR cuts the DNA and the cell can use a similar DNA sequence as a template to try to fix the DNA

• CRISPR changes all PAM's so that they are no longer able to be recognized

A vector in genetic engineering is

• plasmid or virus that contains or carries modified genetic material

• a host organism that is used to grow up lots of copies of transfected DNA

• an organism that can carry a disease to a host

• CRISPR is an example

CRISPR can change DNA in humans and animals.  What did scientists look at to help them create CRISPR?

• Sheep cloning
• Palindromic repeats
• Common Cold
• Bacteria fighting off viruses

What is one way CRISPR has already been used to change genes?

• Clone sheep
• Give people superpowers
• Cure animal diseases
• Military purposes

What enzyme is used in CRISPR gene editing?

• Cas3

• Cpr 9

• Rna5

• Cas 9