PCR & Gel Electrophoresis

PCR & Gel Electrophoresis

12th Grade

8 Qs

quiz-placeholder

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PCR & Gel Electrophoresis

PCR & Gel Electrophoresis

Assessment

Quiz

Science, Biology

12th Grade

Medium

Used 77+ times

FREE Resource

8 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which is the correct order for the PCR process

denaturation, elongation, annealing

annealing, elongation, denaturation

denaturation, annealing, elongation

annealing, denaturation, elongation

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Process of determining the precise order of nucleotides within a segment of DNA

Gel electrophoresis

Polymerase chain reaction

DNA sequencing

DNA amplification

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is PCR used for

genetic testing

paternity testing

determining evolutionary relationship

DNA profiling

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

In gel electrophoresis, which direction do the DNA fragments move?

negative to negative electrodes

negative to positive electrodes

positive to negative electrodes

the DNA doesn't move, the gel does

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What happens during the denaturing step of a PCR reaction

DNA nucleotides on each backbone are broken apart

A heat tolerant polymerase initiates the copying of each strand of DNA

DNA primers attache to the 3' ends of the target sequence

The DNA double helix is separated into two single strands

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Where is Taq polymerase sourced from

man-made in a laboratory

thermal pools

salt mines

all of the above

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Choose the correct order for the temperature changes that occur during the PCR process

95° - 1 minute / 55° - 1 minute / 72° -2 minutes

95° - 1 minute / 72° -2 minutes / 55° - 1 minute

55° - 1 minute / 95° - 1 minute / 72° -2 minutes

95° - 1 minute / 72° -2 minutes / 52° - 1 minutes

8.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Choose the correct term to match the definition:

short nucleic acid that provides a starting point for DNA synthesis

short tandem repeat

agarose gel

Taq polymerase

primer