Which is the correct order for the PCR process
PCR & Gel Electrophoresis

Quiz
•
Science, Biology
•
12th Grade
•
Medium
Used 77+ times
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8 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
denaturation, elongation, annealing
annealing, elongation, denaturation
denaturation, annealing, elongation
annealing, denaturation, elongation
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Process of determining the precise order of nucleotides within a segment of DNA
Gel electrophoresis
Polymerase chain reaction
DNA sequencing
DNA amplification
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is PCR used for
genetic testing
paternity testing
determining evolutionary relationship
DNA profiling
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In gel electrophoresis, which direction do the DNA fragments move?
negative to negative electrodes
negative to positive electrodes
positive to negative electrodes
the DNA doesn't move, the gel does
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What happens during the denaturing step of a PCR reaction
DNA nucleotides on each backbone are broken apart
A heat tolerant polymerase initiates the copying of each strand of DNA
DNA primers attache to the 3' ends of the target sequence
The DNA double helix is separated into two single strands
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Where is Taq polymerase sourced from
man-made in a laboratory
thermal pools
salt mines
all of the above
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Choose the correct order for the temperature changes that occur during the PCR process
95° - 1 minute / 55° - 1 minute / 72° -2 minutes
95° - 1 minute / 72° -2 minutes / 55° - 1 minute
55° - 1 minute / 95° - 1 minute / 72° -2 minutes
95° - 1 minute / 72° -2 minutes / 52° - 1 minutes
8.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Choose the correct term to match the definition:
short nucleic acid that provides a starting point for DNA synthesis
short tandem repeat
agarose gel
Taq polymerase
primer
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