PCR & Gel Electrophoresis

Which is the correct order for the PCR process

• denaturation, elongation, annealing

• annealing, elongation, denaturation

• denaturation, annealing, elongation

• annealing, denaturation, elongation

Process of determining the precise order of nucleotides within a segment of DNA

• Gel electrophoresis

• Polymerase chain reaction

• DNA sequencing

• DNA amplification

What is PCR used for

• genetic testing

• paternity testing

• determining evolutionary relationship

• DNA profiling

In gel electrophoresis, which direction do the DNA fragments move?

• negative to negative electrodes

• negative to positive electrodes

• positive to negative electrodes

• the DNA doesn't move, the gel does

What happens during the denaturing step of a PCR reaction

• DNA nucleotides on each backbone are broken apart

• A heat tolerant polymerase initiates the copying of each strand of DNA

• DNA primers attache to the 3' ends of the target sequence

• The DNA double helix is separated into two single strands

Where is Taq polymerase sourced from

• man-made in a laboratory

• thermal pools

• salt mines

• all of the above

Choose the correct order for the temperature changes that occur during the PCR process

• 95° - 1 minute / 55° - 1 minute / 72° -2 minutes

• 95° - 1 minute / 72° -2 minutes / 55° - 1 minute

• 55° - 1 minute / 95° - 1 minute / 72° -2 minutes

• 95° - 1 minute / 72° -2 minutes / 52° - 1 minutes

Choose the correct term to match the definition:

short nucleic acid that provides a starting point for DNA synthesis

• short tandem repeat

• agarose gel

• Taq polymerase

• primer