Next Generation Sequencing

NGS consists of the following steps in sequence

• library construction, amplification, sequencing

• sequencing, library construction, amplification

• Amplification, Sequencing and library construction

• none of the above

Emulsion PCR refers to

• amplification of DNA in water

• amplification of DNA in organic solvent

• amplification of DNA in water in oil droplets

• synthesis of DNA in water

Bridge PCR promotes

• clusters of identical strands

• ligation of complementary strands

• synthesis of ssDNA

• cleavage of ssDNA

Ion torrent semiconductor sequencing employs

• detection of H+ ions

• difference in pH

• pH sensor probe to detect pH

• all the above

NGS employs

• chemical degradation

• chain termination

• sequence by synthesis principle

• none of the above

Tags

NGSS.HS-LS3-2

Sequencing method that employs bridge PCR

• 454 pyrosequencing

• sequence by ligation

• semiconductor sequencing

• Reversible sequencing

----------- is the first commercial technique not to use fluorescence and camera scanning

• Ion torrent sequencing

• Reversible sequencing

• 454 pyrosequencing

• SoliD sequencing

SoLiD sequencing employs ------ for detection

• H+

• DNA ligase

• DNA polymerase

• Apurase

AN example of HTS technique includes

• Pyrosequencing

• SOLiD sequencing

• Reversible sequencing

• All the above