What are selectable marker ?

• a radium labelled marker

• a type of sequence

• a resistant gene

• a type of protein

What is the importance of cos sites in cosmids?

• to make cosmids infectious

• to make cosmids competent

• allow DNA to be packaged inside lambda phage heads

• permit replication as plasmid in bacteria

How do you overcome vector religation problem?

• Use polymerase enzyme

• Use restriction enzymes

• Use alkaline phosphatase enzyme

• Use ligase enzyme

The restriction enzyme ECOR1 has the property of?

• Endonuclease activity

• Ligation activity

• Correcting the topology of replicating DNA

• Exonuclease activity

pBR322 is an __________________ vector

• original

• cosmid

• artificial

• natural

Copy number is defined as

• Average number of plasmids per bacterial cell

• Average number of plasmids per bacterial chromosome

• Average number of plasmids per bacterial cell or chromosome

• None of the above

Restriction Enzymes are . . .

• proteins

• DNA

• fats

• carbohydrate

What is a palindrome?

• A sequence that is complementary to the other

• A sequence that reads the same forwards and backwards

• A blunt end resulting from a restriction enzyme

• A sticky end resulting from a restriction enzyme

Restriction Enzymes . . .

• cut DNA at precise locations

• help bacteria to pick up DNA

• move DNA through a gel

• copy DNA

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.