Fluorimetry PA

When molecule absorbed incident electromagnetic radiation it is electron goes excited state. But the electron it is unstable at excited state, this electron will return to ground state. When it ground state it is emitting radiation. This phenomena called ?

• A. Fluorescence

• B. Phosphorescence

• C. A and B

• D. None of this

Increase in intensity of light incident on sample increases ________.

• A. absorbance

• B. wavelength

• C. light incident

• D. fluorescence intensity

What wavelengths can a Vis Spectroscopy detect?

• A. 250-600 nanometers

• B. 380-780 nanometers

• C. 620-750 nanometers

• D. 200 and 800 nanometers

What is the best way to describe fluorimetry ?

• General purpose instrument measures voltage, current and resistance.

• Device used to measure parameters of visible spectrum fluorescence.

• A device capable of counting the individual electrons in an electric current.

• Instrument used for detecting and measuring ionizing radiation.

Which spectroscopy is measure intensity of the FLUORESCENCE of molecule?

• A. IR

• B. NMR

• C. Fluorimetry

• D. All of the above

Which of the following are used as sources in fluorometry?

i. Deuterium discharge lamp

ii. Incandescent wire

iii. Xenon arc lamp

iv. Mercury vapor lamp

• i and ii

• i,ii and iii

• iii and iv

• ii and iv

The purpose of secondary filter in fluorescence spectroscopy is

• Allows only emission radiation

• Allows only excitation radiation

• Allows both excitation and emission radiations

• Allows transmitted radiation

What is Jablonski energy Diagram ?

• Is a diagram that illustrates the electronic states of a molecule

• Its just a diagram.

• Diagram too calculate the light

• Is the diagram that the states are arranged vertically by energy and grouped horizontally by spin multiplicity.

Which are the variant of Fluorimetry instrument ?

• Single beam

• Double Beam

• Spectrofluorometer

• All of the above

How long will light be absorbed when the appropriate frequency is irradiated ?

• 5-10 seconds

• 10-15 seconds

• Less than 5 second

• ∞ Infinity ∞