Intro quiz

Which of these is NOT a step in nuclear transport?

• NLS gets recognized by importin

• Ran-GEF exchanges Ran GDP to Ran GTP

• NLS gets cleaved

• Ran-GAP hydrolysis Ran GTP

What determines whether an ERSS-containing protein will be secreted when the transport vesicle fuses with the cell membrane or whether the ERSS-containing protein will become part of the cell membrane?

• The presence or absence of an ERSS

• The presence or absence of an NLS or NES

• The presence or absence of a KDEL

• The presence or absence of a stop transfer sequence

A signal sequence on the polypeptide chain targets all but which proteins to the rough ER surface?

• Golgi proteins

• Lysosomal proteins

• Mitochondrial proteins

• Plasma membrane proteins

Which of the following is responsible for specifically targeting proteins to the Golgi membrane?

• ERSS

• MP6

• Stop transfer sequence of the correct length

• None of the above

Release of the ERSS from the SRP upon arrival to the translocon does not require energy

• True

• False

Secreted proteins and cellular membrane proteins share these characteristics, EXCEPT for

• Both have a stop transfer sequence

• Both follow the secretory pathway

• Both have an ERSS

Adaptor proteins ____________ and coat proteins____________

• direct budding ; select cargo

• select cargo ; direct budding

• transport the protein ; recognize the target

• recognize the target ; transport the protein

SNAREs function in which step of the life of a vesicle?

• Budding

• Transport

• Docking

• Fusion

Dynein moves in...

• Retrograde (negative) direction

• Retrograde (positive) direction

• Anterograde (negative) direction

• Anterograde (positive) direction

A protein that is found in the membrane domain at point D must have the following signal sequences (select all that apply) :

• NLS

• ERSS

• NES

• MP6

• Stop transfer sequence