RECOMBINANT DNA TECHNOLOGY-PMH

Which is not true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.

• Plasmids are vectors

• Plasmids can contain an antibiotic resistance gene.

• plasmids are made of single strand of DNA

In bacteria, restriction endonucleases are used for defense. They cut up the DNA of a virus that infects bacteria.

• True

• False

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• amplification

• insertion

• transformation

• screening

Which enzyme helps insert the gene of interest into the vector?

• DNA Polymerase

• DNA ligase

• RNA polymerase

• DNA helicase

The cell that receives recombinant DNA for cloning purpose is known as

• vector

• target DNA

• plasmid

• host cell

Bacteria use restriction enzymes to protect themselves from:

• other bacteria

• plants

• antibiotics

• viruses

Tags

NGSS.HS-LS4-4

Which of the following enzymes can hydrolyse phosphodiester bonds?

• DNA polymerase

• restriction endonuclease

• DNA helicase

• reverse transcriptase

Which restriction enzyme listed below was isolated from E. coli bacteria?

• EcoRI

• HindIII

• HaeII

• SmaI

Which of the following is NOT a tool used in genetic engineering?

• plasmid

• restriction enzyme

• DNA helicase

• DNA ligase

What type of cut is shown in this photo?

• Blunt

• sticky