10.8.21XII BLN-4 Q-2

Pick out the bacteria used as a SCP .

• Chlorella

• Spirulina

• Chlamydomonas

• Cellulomonas

Spirulina can be grown easily on materials

like

• waste water from potato processing plants

• straw

• animal manure and even sewage,

• all the above

_____________ are enzymes which remove

nucleotides one at a time from the end of a

DNA molecule.

• DNA Ligase

• Endonucleases

• Exonucleases

• Alkaline Phosphate

___________ are enzymes which break

the internal phosphodiester bonds within a

DNA molecule.

• Exonucleases

• Endonucleases

• HindII

• DNA Ligase

The restriction enzyme __________ always cut DNA molecules at a point of recognising a

specific sequence of six base pairs.

• HaeII

• Hind II

• EcoRI

• Alu I

The first letter of the enzymes indicates the ______

• genus name

• species

• strain

• discovery

Some cleave both strands of DNA through the centre resulting in ______

• blunt end

• flush end

• sticky end

• both a and b

Some enzymes cut in a way producing protruding and recessed ends known as ____

• sticky end

• cohesive end

• blunt end

• both a and b

________ is a DNA modifying enzymes and adds

or removes specific phosphate group at 5’

terminus of double stranded DNA (dsDNA) or

single stranded DNA (ssDNA) or RNA.

• DNA ligase

• Alkaline Phosphatase

• EcoRI

• Hindii

Pick out the palindromic sequence.

• 5’ ... CATTATATAATG ... 3’

  3’ ... GTAATAATTTAC ... 5’

• 5’ ... CTATATATAATG ... 3’

  3’ ... GTAATATATTAC ... 5’

• 5’ ... CATTATATAATG ... 3’

  3’ ... GTAATATATTAC ... 5’

• 5’ ... CATTATATAAGT ... 3’

  3’ ... GTAATATATTAC ... 5’