Expression of Biological Information

What is meant by description "antiparallel" regarding the strands that make up DNA?

• The twisting nature of DNA creates nonparallel strands.

• The 5' to 3' direction of one strand runs counter to the 5' to 3' direction of other strand.

• Base pairings create unequal spacing between the two DNA strands.

• One strand is positively charged and the other is negatively charged.

An Okazaki fragment has which of the following arrangements?

• Primase, DNA polymerase, DNA ligase

• 3' RNA nucleotides, DNA nucleotides 5'

• 5' RNA nucleotides, DNA nucleotides 3'

• 5' DNA to 3'

In E.coli, there is a mutation in a gene called DNA B that alters the helicase that normally acts at the origin. Which of the following would you expect as a result of this mutation?

• No replication fork will be formed.

• No proofreading will occur.

• The DNA will supercoil.

• Replication will require a DNA template from another source.

Which enzyme catalyzes the elongation of DNA strand in the 5' to 3' direction?

• Primase

• Topoisomerase

• DNA polymerase III

• DNA polymerase I

Which property of DNA enables it to replicate semi-conservatively?

• covalent bond between bases and sugar groups

• covalent bond between bases and phosphate groups

• covalent bond between components

• hydrogen bond between bases

DNA Replication produces

• RNA molecules

• only new DNA

• 2 DNA molecules, one old strand and one new strand

• excessive DNA which eventually causes the nucleus to divide

What is the function of primase?

• forming replication fork

• unwinding the DNA double strand to allow DNA polymerase to function

• hydrolyzing ATP to facilitate DNA unwinding

• making short strands of RNA at the site where replication begins

What are segments labelled P and Q?

• P: template and Q: Lagging strand

• P: Leading strand and Q: RNA primer

• P: Lagging strand and Q: Okazaki fragment

• D: Leading strand and Q: Okazaki fragment

Which process is NOT involved in protein synthesis?

• elongation

• replication

• translation

• transcription

Which enzyme involves in protein synthesis?

• DNA ligase

• DNA polymerase

• RNA polymerase

• DNA helicase