Gene cloning revision (1)

The diagram shows the structure of E.coli, which is gram negative. How can the cell extract be obtained?

• Add EDTA, lysozyme, and SDS.

• Add lysozyme and SDS.

• Add EDTA and SDS.

• Add EDTA and lysosyme.

From the diagram, we can conclude that...

• low salt concentration causes DNA to elute from the column.

• DNA binds most tightly to the ion-exchange resin.

• RNA and protein bind most tightly to the ion-exchange resin.

• a single salt concentration can separate DNA from RNA and protein.

This diagram shows...

• how plasmids are separated from chromosomal DNA by size

• the alkaline denaturation method

• how plasmids are separated from chromosomal DNA by conformation

• all of the above

this diagram shows the action of...

• terminal transferase

• alkaline phosphatase

• polynucleotide kinase

• the klenow fragment

in the diagram shown, what is enzyme A?

• DNA ligase

• DNA polymerase II

• Klenow fragment

• Taq polymerase

in homopolymer tailing using terminal transferase, the issues shown may result. how can these be solved?

• ligase and DNA polymerase I

• klenow fragment and restriction endonuclease

• restriction endonuclease and polynucleotide kinase

• ligase and klenow fragment

How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that may apply.

• Deoxyribonucleotides are used in both PCR and Cycle (Chain termination) Sequencing.

• Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.

• You cannot add new nucleotides to a deoxyribonucleotide.

• Deoxyribonucleotides are NOT found in natural living cells.

• Dideoxyribonucleotides are labeled with fluorescent tags.

How is DNA synthesis in PCR and Sanger's Chain Termination Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

How is DNA synthesis in PCR and Sanger's Chain Termination Sequencing DIFFERENT?

• Only PCR uses deoxynucleotides.

• Cycle Sequencing generates DNA products of different lengths.

• Only PCR analyzes DNA products using gel electrophoresis.

• Only Cycle Sequencing uses dideoxynucleotides.

What do dideoxynucleotides do when added to a growing DNA strand during Sanger's Chain Termination Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.