Molecular Techniques

On average, which restriction nuclease would cut a random DNA sequence into a greater number of pieces?

• That recognises a four nucleotide sequence

• That recognises eight nucleotide sequence

Gel electrophoresis can be used to separate DNA fragments by virtue of which feature?

• Charge

• Size

• Sequence

When a mixture of DNA fragments of different sizes is loaded onto an agarose gel and a voltage is applied, the DNA will migrate in the gel. Which molecules will migrate the fastest?

• The shortest

• The longest

• Molecules with highest fraction of A/T nucleotides

• Molecules with highest fraction of G/C nucleotides

When DNA fragments are being separated by gel electrophoresis, toward which electrode do the DNA fragments move?

• The positive electrode

• The negative electrode

What does the term DNA cloning refer to?

• Cutting DNA with restriction nucleases

• Putting a foreign gene into a cell

• Making an identical copy of a DNA molecule

When making DNA from starting material of mRNA which enzyme is required to copy the mRNA molecules?

• RNA polymerase

• DNA ligase

• Reverse transcriptase

• DNA polymerase

In order to design primers for PCR, which part of the sequence do you need to know?

• Full sequence

• The ends of the DNA sequence of interest

• The mid-region of the DNA sequence of interest

In the polymerase chain reaction, what is used to separate the two strands of double stranded DNA?

• Heat

• Excess concentration of primers

• pH

Which of the following techniques allows the study of gene expression of thousands of genes of interest?

• Southern Blotting

• DNA microarrays

• DNA sequencing

• IHC

Which of the following is TRUE

• RNA interference can block RNA transcription

• RNA interference is based on a natural mechanism many organisms use to avoid infection.

• RNA interference is a quick and easy way to activate a target gene.