Electrophoresis and Chromatography

What is the first stage of the two-stage two-dimensional PAGE?

• SDS-PAGE

• HPLC

• Isoelectric focusing

• Sedimentation

Gel electrophoresis is used to separate DNA fragments on the basis of which property?

• Size

• Color

• Functions

• Chromosomes

In gel electrophoresis, how do we make the DNA migrate through the gel?

• We place a negative electrode on the bottom and away from the wells.

• Gravity

• We place a positive electrode on the bottom and away from the wells.

• Large fragments drift to the end of the gel.

Identify the correct statement about a gel filtration column.

• Smaller proteins enter the beads easily

• Large proteins are eluted first

• Large proteins enter the beads easily

• Smaller proteins enter the beads easily causing large proteins to be eluted first

The following statements are true about SDS-PAGE, except for one.

• Disulfide bonds are cleaved by beta-mercaptoethanol.

• Proteins are denatured by the SDS.

• Proteins will have a positive charge because of SDS attachment.

• Larger proteins move more slowly.

In a native PAGE, proteins are separated on the basis of ______.

• net negative charge

• net charge and size

• net positive charges and size

• net positive charge

Which of the following must be added to the acrylamide solution to prepare the gel?

• TEMED and APS

• Tris-EDTA

• SDS and BME

• Coomassie blue

Which of the following statements about agarose gel electrophoresis is False?

• Electrophoresis in agarose is a common way to separate DNA molecules according to charge.

• Agarose gel is extracted from seaweeds.

• Under the influence of the electrical field, positively charged DNA will migrate towards the negative charged end of the gel.

• None of them

Which of the following statements about agarose gel electrophoresis is False?

• Electrophoresis in agarose is a common way to separate DNA molecules according to charge.

• Agarose gel is extracted from seaweeds.

• Under the influence of the electrical field, positively charged DNA will migrate towards the negative charged end of the gel.

• None of them

Which of the following statements about agarose gel electrophoresis is False?

• Electrophoresis in agarose is a common way to separate DNA molecules according to charge.

• Agarose gel is extracted from seaweeds.

• Under the influence of the electrical field, positively charged DNA will migrate towards the negative charged end of the gel.

• None of them