DNA replication and PCR

This image shows that DNA Replication is _______.

• Conservative
• Semiconservative
• Dispersive
• Spontaneous

What part of a nucleotide accounts for the genetic variation between individuals?

• phosphate
• nitrogenous base
• deoxyribose
• sugar

What is the purpose of PCR?

• To produce millions of copies of the DNA genome

• To produce millions of copies of a specific region of DNA

• To add nucleotides to a DNA sequence

• To watch polymerase work.

What does PCR stand for?

• Polymerase Cytoplasmic Reaction

• Polymerase Chromosomal Reaction

• Polymerase Chain Reaction

• Probably Cannot React

What happens in the denature step of PCR?

• The DNA nucleotides are broken apart.

• The base-pairing rules for DNA are reversed.

• The double-stranded DNA is separated into two single strands of DNA.

• The DNA is returned to its natural setting.

At what temperature does the denature step of PCR occur?

• 94-96° C

• 50-65° C

• 72° C

• Any temperature

At what temperature does the anneal step of PCR occur?

• 94-96° C

• 50-65° C

• 72° C

• Any temperature

What happens during the anneal step of PCR?

• Primers are created.

• The primers attach to the target DNA region.

• Primers copy the new DNA strand.

• Primers sequence DNA

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.

• To copy DNA.

• To create DNA nucleotides.

• To maintain the temperature of the PCR reaction.

What is the purpose of the extension step of PCR?

• To create the primers.

• To extend the time it takes to produce DNA.

• To allow polymerase to create the complementary strands of DNA.

• To allow substrates to copy DNA sequences.