Recombinant DNA

What is recombinant DNA?

• The altering of genetic material.

• When DNA from two different organisms is joined.

• An exact copy of a DNA segment.

• Small rings of DNA

Tags

NGSS.HS-LS3-2

What steps are involved in 'recombining' DNA?

• Isolate DNA

• Cut DNA with restriction enzyme

• Transfer DNA to nylon membrane

• Expose x-ray film to radiolabeled membrane.

• Mix DNAs and join them using DNA ligase.

What is a clone?

• An exact copy of a DNA segment.

• DNAs that can replicate within a cell.

• A strand of RNA labeled with a radioactive element.

• Variations in the length of DNA.

What is a cloning vector?

• An agent used to transfer DNA.

• Small rings of DNA.

• An organisms complete set of proteins.

• Something used to do DNA fingerprint.

What is step 1 of 'recombining' DNA?

• Allowing the bacterium to reproduce.

• Mixing the DNAs together using DNA ligase.

• Cutting the DNA with a restriction enzyme.

• Isolating the DNA.

What is the second step in 'recombining' DNA?

• Isolating the DNA.

• Cutting the DNA with a restriction enzyme.

• Inserting the recombinant plasmid into a host bacterium.

• Allowing the bacterium to reproduce.

Third step in 'recombining' DNA?

• Mixing the DNAs together and joining them using DNA ligase.

• Isolating the DNA.

• Inserting the recombinant plasmid into the host bacterium.

• Allowing the bacterium to reproduce.

Fourth step in 'recombining' DNA?

• Cutting DNA with a restriction enzyme.

• Isolating the DNA.

• Allowing the bacterium to reproduce.

• Inserting the recombinant plasmid into a host bacterium.

Last step in 'recombining' DNA?

• Allowing the bacterium to reproduce.

• Isolating the DNA.

• Mixing the DNAs together and joining them using DNA ligase.

• Cutting the DNA with a restriction enzyme.

What is a probe?

• A tool that makes DNA fingerprints more accurate.

• A strand of RNA or a single-stranded DNA; labeled with a radioactive element or florescent dye.

• An exact copy of DNA, a whole cell, or a complete organism.

• Variations in the length of the DNA molecule between known genes.

Tags

NGSS.HS-LS3-2