Genetic Technology

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

What happens in the Denature step of PCR?

• The DNA nucleotides are broken apart.
• The base-pairing rules for DNA are reversed.
• The double-stranded DNA is separated into two single strands of DNA.
• The DNA is returned to its natural setting.

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

What is the Extension step?

• DNA polymerase adds nucleotides to the 5' end

• RNA polymerase adds nucleotides to the 5' end

• DNA polymerase adds nucleotides to the 3' end

• RNA polymerase adds nucleotides to the 3' end

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

What is the best definition for "primer"?

• An oligonucleotide of unknown sequence that is used to amplify the target gene.

• An oligonucleotide of known sequence that is used to amplify the target gene.

• An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.

• A section of DNA on the target gene that is the binding site for Taq polymerase.

During which PCR step do primers form complementary base pairs with single-stranded template DNA?

• Denaturation

• Annealing

• Extension

• Synthesis

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

• DNA polymerase

• Primers

• Deoxyribonucleotides (dNTPs)

• Dideoxyribonucleotides (ddNTPs)

• DNA template