Endonucleases, ligases and polymerases

Which type of enzyme act as genetic scissors?

• Ligases

• Restriction Enzymes

• Endonucleases

• Polymerases

Which enzyme acts as genetic glue?

• Ligases

• Restriction enzymes

• Endonucleases

• Polymerases

Which enzymes act to build polymers from their monomers?

• Ligases

• Restriction enzymes

• Endonucleases

• Polymerases

Which suffix is most commonly found on the name of an enzyme?

• -ide

• -ase

• -ose

• -itis

An endonuclease has cut a fragment of DNA directly across. Which end is the result?

• Sticky-end

• Blunt-end

An endonuclease has cut a fragment of DNA in a staggered manner. Which end is the result?

• Sticky-end

• Blunt-end

Which type of cut DNA is easier to work with?

• Sticky-end

• Blunt-end

Explain why blunt-end DNA fragments are harder to work with in biotechnology.

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Which type of enzyme can build DNA molecules?

• RNA Polymerase

• DNA Ligase

• DNA Endonuclease

• DNA Polymerase

Explain how endonucleases and ligases work well together.

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