Photometric Measurement Part 2

What is the principle of turbidimetry?

• It measures the concentration of a solution based on fluorescence

• It measures the amount of scattered light

• It measures light absorption by a cloudy solution

• It measures emitted light from excited atoms

Which of these is not associated with the use of nephelometry?

• measures drug levels

• to quantitate metabolites like bilirubin

• measures serum proteins

Which component is unique to nephelometry compared to spectrophotometry?

• Photocell

• Laser light sources

• Cuvette

• Monochromator

What is the main principle behind fluorometry?

• It measures light absorption

• It measures emitted light from excited atoms

• It measures the molecule concentration based on fluorescence intensity

• It measures scattered light

Which of the following is a disadvantage of fluorometry?

• It is very sensitive

• Not all molecules fluoresce

• It is less sensitive than spectrophotometry

• It is ideal for measuring proteins

What is the role of the primary filter in fluorometry?

• To detect emitted light

• To control light intensity

• To select the desired wavelength

• To hold the sample

What is the main principle of atomic emission spectrophotometry?

• It measures fluorescence intensity

• It measures scattered light

• It measures emitted light from excited atoms

• It measures light absorption by molecules

Which gas is commonly used in atomic emission spectrophotometry?

• Nitrogen

• Hydrogen

• Oxygen

• Acetylene

What is the purpose of an internal standard in atomic emission spectrophotometry?

• To increase sensitivity

• To stabilize measurements

• To measure light absorption

• To enhance fluorescence

What is a common disadvantage of atomic absorption spectrophotometry?

• It is free from spectral interference

• It provides good sensitivity

• Chemical interference can occur

• It is ideal for measuring proteins