Unit 7 Review

Analyze the electrophoresis gel depicting the DNA of 4 suspects (lanes 1-4) and DNA found at a crime scene (lane CS).

Which suspect most likely committed this crime?

• Suspect 1

• Suspect 2

• Suspect 3

• Suspect 4

Review the DNA sequence of an individual.

If this sequence was prepared for gel electrophoresis with a restriction enzyme which recognized the sequence AAT, how many bands would compose the DNA fingerprint for the individual?

• one band

• two bands

• three bands

• four bands

During gel electrophoresis, what is applied to the negatively charged DNA fragments to separate them on the basis of their size?

• ultraviolet radiation

• electric current

• magnetic force

• x-ray radiation

Gel electrophoresis is a laboratory technique designed to separate molecules such as DNA, RNA, or proteins based upon what property?

• number of amino acids

• number of fatty acids

• shape

• size

Which sequence correctly identifies the steps in bacterial transformation?

• 1) insert gene into bacterial plasmid; 2) confirm the plasmid has been taken in; 3) select transformed bacteria; 4) produce the desired product

• 1) produce the desired product; 2) insert gene into bacterial plasmid; 3) confirm the plasmid has been taken in; 4) select transformed bacteria

• 1) select transformed bacteria; 2) insert gene into bacterial plasmid; 3) confirm the plasmid has been taken in; 4) produce the desired product

• 1) select transformed bacteria; 2) confirm the plasmid has been taken in; 3) insert gene into bacterial plasmid; 4) produce the desired product

The diagram shows a segment of bacterial DNA that is cut using a restriction enzyme.  Which plasmid DNA fragment can be used to insert the bacterial DNA segment?

• 
• 
• 
• 

Which statement explains why bacteria are commonly used as vectors in genetic engineering?

• They can synthesize and match the replication enzymes of almost all the organisms.

• They have a short replication time, so any DNA inserted in them can be quickly multiplied.

• They have a foolproof method of replication that produces copies of DNA without any errors.

• They have a slow replication process, so DNA can be altered in the intermediate replication steps.

What was one of the original purposes of the Human Genome Project?

• to locate every gene on each human chromosome

• to create plasmids for transferring DNA between humans

• to sequence human mitochondrial DNA which is passed on from only a mother to her child

• to compare human mitochondrial DNA with chloroplast DNA from large dicot plants

Researchers are in the process of developing a tooth bud using adult dental stem cells that when implanted in the gums, will replace damaged or lost teeth in humans. This bioengineered tooth will be fully functional like a normal tooth.

Which statement describes a benefit of the bioengineered tooth?

• It will not decay like a normal tooth.

• It will help the recipient bite food more easily than a normal tooth.

• It will be stronger than a typical tooth.

• It will be readily accepted by the recipient’s body.

10.In the field of medicine, researchers have recently designed a technique that allows them to use adult stem cells to test the potency and safety of newly designed drugs for humans.

What is the most likely advantage of this new technology compared with other forms of drug trials?

• It helps ensure that the shelf life of newly designed drugs is as long as possible.

• It helps ensure that the newly designed drugs can cure all patients they are used to treat.

• It helps reduce the demand to test newly designed drugs on animals.

• It helps reduce the time it takes to bring newly designed drugs to the market.