Enzyme Kinetics

What is the meaning of these terms: [E], [S], [ES]

• Respectively for concentration of enzyme, concentration of substrate, and concentration of enzyme-substrate complex

• Respectively for amount of enzyme, concentration of substrate, and concentration of enzyme-substrate complex

Which statements is about competitive inhibitor?

• Binds to the allosteric site of enzyme

• Binds permanently to the enzyme

• The effect of enzyme can be overcome by adding more substrates

• It will cause denaturation of enzyme

Tight binding interaction of enzyme substrate indicates ......... Km value

• Large

• Initially large and then small

• Initially small and the large

• Small

In which type of Inhibition does the inhibitor bind with ES complex

• Uncompetitive

• Non competitive

• Competitive

• All of the above

Bi-substrate reactions involving formation of a tertiary complex are

• Sequential

• Competitive

• Ping Pong

• None of these

The graph shown here represents which type of bi-substrate reaction

• Sequential Ramdon

• Sequential Ordered

• Ping Pong

• None of these

Competitive Inhibitors bind to

• Substrates

• ES Complex Only

• ES Complex or Free enzyme (E)

• Free enzyme only (E)

Which of the following is true of competitive inhibition?

• Vmax doesn't change; Km decreases

• Vmax goes down; Km increases

• Vmax doesn't change; Km decreases

• Vmax goes down; Km decreases

Uncompetitive inhibitors bind to

• ES or E

• E only

• ES only

• None of these

Which type of inhibition is demonstrated on the graph?

• Competitive

• Uncompetitive

• Mixed

• Not enough information