BD Rhapsody Internal Training

What does BD Rhapsody™ Single-Cell Analysis System consists of?

• BD Rhapsody™ Scanner

• BD Rhapsody™ Express System

• BD Rhapsody™ P1200M and P5000M pipettes

• BD Rhapsody Cartridge

BD™ Single-Cell Multiplexing Kits allow researchers to pool up to __ samples to reduce time and cost, as well as minimize batch effects.

Which of the following single-cell multiomics reagents is not available by BD?

• AbSeq oligos

• Mouse Immune Response Targeted Panel 

• Whole Transcriptome Analysis (WTA) Amplification Kits

• None of the above

Which of the following is the usage of this Scanner Analysis Metric:

'Number of wells with viable cells and a bead'

• Estimation of occurrence of multiple cells captured by the same bead in a well

• Estimation of the number of wells with viable cells captured with beads

• Estimates the total number of viable cells captured on a bead at cell lysis

• Indicates if the cartridge is significantly underloaded with beads

Which of the following is the right order of BD Rhapsody single cell workflow?

1. Synthesize cDNA

2. Lyse cells

3. Retrieve beads

4. Analyze data

5. Load cells

6. Load beads

• 6 > 5 > 2 > 1 > 3 > 4

• 6 > 5 > 2 > 3 > 1 > 4

• 5 > 6 > 2 > 3 > 1 > 4

• 5 > 2 > 6 > 1 > 3 > 4

Which of the following are the advantages of BD Rhapsody single cell analysis systems?

• Broad range of cell throughput (100 - 40,000 cells per cartridge)

• 2-3 % multiplet rate at 10,000 cell load

• Repeat experiment can be done with cell capture beads archived for up to 3 months

• Confidence with every experiment with visual workflow QC on Rhapsody scanner

• No sample loss and clogging issue with microwell technology

Which of the following items are required for BD targeted single cell mRNA library preparation and are unavailable by BD?

• Calcein AM

• Agencourt® AMPure® XP magnetic beads

• Targeted mRNA and AbSeq Reagent Kit

• Qubit™ dsDNA HS Assay Kit

The following targeted assays are available by BD, except

• Onco BC panel

• B cell panel

• TCR/BCR 

  Multiomic Assay

• Custom panel

In the BD Rhapsody cartridge, cells settle into the micro-wells by __?

The Single Cell Multiplexing kit can be used interchangeably for both human and mouse samples.

• True

• False