CHEM 570 - Ch28

What is HPLC?

• A technique to separate components based on pH

• A technique to separate components based on polarity

• A technique to separate components based on liquidity

• A technique to separate components based on temperatue

C18 and silica are stationary phased that used in ...

• Normal and Normal HPLC respectively

• Normal and Reverse HPLC respectively

• Reverse and Normal HPLC respectively

• Reverse and Reverse HPLC respectively

What is the function of degasser?

• To add gas into the system of HPLC

• To prevent gas into the system of HPLC

• To change liquid into gas

• To change gas into liquid

What is the common pump used in HPLC?

• Syringe pump

• Plunger pump

• Diaphragm pump

Which substance is used in normal phased HPLC?

• stationary phase: high polar

  mobile phase: low polar

• Stationary phase: high polar

  Mobile phase: high polar

• Stationary phase: low polar

  Mobile phase: low polar

• Stationary phase: low polar

  Mobile phase: high polar

Which eluent will be eluted first in reverse phase of HPLC?

• The least polar

• The highest polar

• The simplest compound

• The most complex compound

Solid phase extraction is used to ...

• purify the samples from unwanted components

• purify the solvents from unwanted components

• purify the samples using low elution strength

• purify the samples using high elution strength

Water / methanol is used as a mobile phase in ...

• Normal phase of HPLC

• Reverse phase of HPLC

The ability of an analytical procedure to remain unaffected by small changes is called ...

• Linearity

• Robustness

• Quantitation Limit

• Range

In which temperature the column oven has to be maintained?

• 25-50 degrees

• 30-50 degrees

• 35-60 degrees

• 40-60 degrees