DNA Replication

Statement I: The strand with polarity 3'-5' only acts as the template during DNA replication.

Statement II: The newly synthesized strand is polymerized in 3'-5' polarity

• Only statement I is false

• Only statement I is true

• Both the statements are true

• Both the statements are false

The enzyme responsible for formation of RNA primer on lagging strand during DNA replication is

• Primase

• DNA depedent DNA polymerase

• DNA dependent RNA Polymerase

• Both A and C

The unwinding of DNA helix at ORI region is done by

• Primase

• Ligase

• Helicase

• SSBP

Which of the following is not correct in relation to topoisomerase II?

• It cut both the strands of DNA at same time and ligate them after removing supercoiling

• Its function is to remove supercoiling in front of replication fork

• It always work in association with RNA primase

• It relieves the tension from the DNA generated due to unwinding of by helicase

DNA replication,

• Occurs during the S and G0 phase of Interphase

• Is associated with the condensation of DNA into Chromosomes

• is an enzyme mediated process that occurs in both prokaryotes and eukaryotes

• is more prone to errors as enzymes used are not specialized for proofreading

E. coli DNA Polymerase I differs from DNA polymerase III in that the former has

• 3'-5' exonuclease activity

• 5'-3' exonuclease activity

• 5'-3' polymerisation activity

• 3'-5' DNA reading activity

The discontinuously synthesized strand,

• is polymerized in 3'-5' direction

• is polymerized entirely by DNA Pol. I

• is also called as leading strand

• is the one which possess okazaki fragments.

Identify the mismatch pair.

• DNA Ligase - joins discontinuous framents later

• Primase - formation of okazaki fragments

• DNA Polymerase III - Polymerization of new daughter strand

• SSBP - Stabalizes single strand DNA of replication fork

Which of the following is correct with respect to DNA replcation?

• Enzyme Helicase is used to stabilize the ssDNA s

• Enzyme ligase is used to employ RNA primers

• Enzyme DNA Pol. I is the main polymerizing enzyme

• Okazaki fragments are made up of deoxynucleotides

For the strand separation and stabilisation during DNA replication which of the following set of enzymes and proteins are required?

• SSBP, gyrase, primase

• Topoisomerase, helicase, ligase

• Gyrase, ligase, primase

• Topoisomerase, helicase, SSBP