Enumeration and Isolation of Bacteria

If i spot plated a dilution series and came in the next day and saw that my 10^-6 dilution quadrant had 3, 2 and 4 colonies, I would then:

• Not use for calculation, TFTC

• Not use for calculation, TNTC

• Perform a cfu/mL calculation

If i need to transfer a 325 uL solution into a test tube, I would use a :

• P20

• P200

• P1000

• graduated cylinder

A technique that utilizes an inoculating loop and bunsen burner to transfer bacteria from one medium to another in an organized fashion that results in isolated colonies is called ______

If you want to determine how much bacteria is in a culture, which of the following would you use

• Streak for Isolation

• Spread plating

• Spot plating

• Spread or Spot plating

• Streak for isolation, Spread and spot plating

Examples of aseptic technique include:

• Wearing a lab coat, goggles and gloves

• Flaming inoculating loops and needles before and after use

• Keeping lids on agar plates between uses

• All of the above

A group of millions of genetically identical cells that are stacked up on top of each other are referred to as

• cells

• colonies

• cultures

• spots

Put the following postulates in the correct order

I. Isolate microorganism and grow in a lab setting

II. Inject a healthy subject with isolated microorganism and notice it becomes sick

III. observe microorganism in a sick subject not found in a healthy subject

IV. Re-isolate identical microorganism found in sick subject

• I, III, IV, II

• III, I, II, IV

• I, II, III, IV

• III, I, IV, II

The theory that proposes that microorganisms are the cause of many diseases is called _____

Form, elevation, and margin descriptions would be used when referring to ______ morphology

Perform a cfu/mL calculation with a countable spread plate. Choose the correct cfu/ml below

Plate 1: 175 colonies, 100 uL plated, 10^-5 dilution

Plate 2: 22 colonies, 150 uL plated, 10^-7 dilution

Plate 3: 402 colonies, 150 uL plated, 10^-3 dilution

• 1.92 x 10^3 cfu/mL

• 1.75 x 10^8 cfu/mL

• 1.92 x 10^8 cfu/mL

• 8.47 x 10^7 cfu/mL