Enumeration and Isolation of Bacteria

If i spot plated a dilution series and came in the next day and saw that my 10^-6 dilution quadrant had 3, 2 and 4 colonies, I would then:

• Not use for calculation, TFTC

• Not use for calculation, TNTC

• Perform a cfu/mL calculation

If i need to transfer a 325 uL solution into a test tube, I would use a :

• P20

• P200

• P1000

• graduated cylinder

A technique that utilizes an inoculating loop and bunsen burner to transfer bacteria from one medium to another in an organized fashion that results in isolated colonies is called (a)  

If you want to determine how much bacteria is in a culture, which of the following would you use

• Streak for Isolation

• Spread plating

• Spot plating

• Spread or Spot plating

• Streak for isolation, Spread and spot plating

Examples of aseptic technique include:

• Wearing a lab coat, goggles and gloves

• Flaming inoculating loops and needles before and after use

• Keeping lids on agar plates between uses

• All of the above

A group of millions of genetically identical cells that are stacked up on top of each other are referred to as

• cells

• colonies

• cultures

• spots

Put the following postulates in the correct order

I. Isolate microorganism and grow in a lab setting

II. Inject a healthy subject with isolated microorganism and notice it becomes sick

III. observe microorganism in a sick subject not found in a healthy subject

IV. Re-isolate identical microorganism found in sick subject

• I, III, IV, II

• III, I, II, IV

• I, II, III, IV

• III, I, IV, II

The theory that proposes that microorganisms are the cause of many diseases is called (a)  

Form, elevation, and margin descriptions would be used when referring to (a)   morphology

Perform a cfu/mL calculation with a countable spread plate. Choose the correct cfu/ml below

Plate 1: 175 colonies, 100 uL plated, 10^-5 dilution

Plate 2: 22 colonies, 150 uL plated, 10^-7 dilution

Plate 3: 402 colonies, 150 uL plated, 10^-3 dilution

• 1.92 x 10^3 cfu/mL

• 1.75 x 10^8 cfu/mL

• 1.92 x 10^8 cfu/mL

• 8.47 x 10^7 cfu/mL