Proteins MT6310 Lab 2DMT

Write any question that you have in mind about the given experiment on Proteins? Do not leave it blank. Do not type none or n/a or i have no questions mam.

Large complex molecules critical for physiological homeostasis and function

• amino acids

• peptide bonds

• proteins

• enzymes

small units that made up proteins

• amino acids

• peptide bonds

• sugars

• enzymes

attachment of proteins is made possible by

• amino acids

• peptide bonds

• sugars

• enzymes

Click all the aromatic amino acids

• Phenylalanine

• Tyrosine

• Tryptophan

• Lysine

• Glutamate

This is a protein assay that works on absorption of 280nm light by aromatic residues

• Spectrophotometric

• Bicinchonic Acid Protein Assay

• Bradford Assay

• Lowry Method

• Biuret Assay

This is a protein assay that works on Coomassie dye that shifts absorption when bound to proteins

• Spectrophotometric

• Bicinchonic Acid Protein Assay

• Bradford Assay

• Lowry Method

• Biuret Assay

This is a protein assay that works on Biuret reaction and copper complexes

• Spectrophotometric

• Bicinchonic Acid Protein Assay

• Bradford Assay

• Lowry Method

• Biuret Assay

This is a protein assay that gives a purple complex result when peptide bonds reacts with alkaline copper

• Spectrophotometric

• Bicinchonic Acid Protein Assay

• Bradford Assay

• Lowry Method

• Biuret Assay

Reorder the following steps in chromatography

• Visualization

• Sample and Standard Application

• Evaluation

• Development

• Documentation