Unit 1.1 Mystery Diagnosis Exam

What category of medical intervention would vitamins, vaccines, and helmets fall under

• Treatment

• Prevention

• Diagnosis

• Therapeutic

When determining the genetic sequence of an organism; PCR is used to duplicate DNA, enzymes cut it into different sized pieces, electrophoresis is used to sort it and laser is used to

• read the genetic sequence based on fluorescent nucleotides

• cut the DNA fragments into individual genes segments

• calculate the speed at which the DNA fragments move through the gel

• All of these

While working in a pathogen lab an epidemiologist asks you to run a BLAST, what are they expecting you to do?

• Search a national database to match the genome sequence of the sample

• Use restriction enzymes to cut DNA fragments into smaller and smaller pieces

• Use hot vapor to disinfect a room contaminated by an unknown pathogen

• Determine what anitbodies are needed to deactivate a specific antigen

What does it mean to 'amplify' a DNA sample

• Recombine DNA using restriction enzymes

• Grow bacteria that host that DNA fragment in a petri dish

• Use PCR to make many more copies

• Run the DNA fragment through a sequence to get the genome

B cells produce uniquely shaped proteins called antibodies that bind to ______ to mark them for destruction.

• Enzymes

• Secondary Antibodies

• Antigens

• Hormones

When using an ELISA test to determine if a patient tests positive for a disease a fluid sample from the patient is used. If the patient is positive for the disease the _____ will bind to the test well first.

• Antibody

• Antigen

• Pathogen

• Secondary Antibody

What is the role of the substrate in an ELISA test?

• It binds directly to the antigen if the antigen is present

• It washes excess fluid away from the antibodies to the antigen can bind

• It binds to the secondary antibody and causes a color change

• No substrate is used in an ELISA

Which is the correct sequence when performing an ELISA

• substrate > antigen > primary antibody > secondary antibody > enzyme

• primary antibody > secondary antibody >substrate > antigen > enzyme

• secondary antibody >substrate > antigen > primary antibody > enzyme

• antigen > primary antibody > secondary antibody > enzyme > substrate

The results of ELISA may vary based on concentration of

• antigen present

• secondary antibody

• primary antibody

• substrate

Tags

NGSS.HS-PS1-5

This ELISA samples show different tints of blue. What can we infer about the patients who's test resulted in light tinted blues?

• They are likely sickest because this shows their body isn't making many antibodies

• They are probably getting over being sick because the light color indicates they have very few antigens

• We can ONLY determine that they have a lower number of antigens

• We can ONLY determine that they have the most antibody activity