Thin Layer Chromatography

TLC stands for...

• Tender Love & Care

• Thick Layer Chromatography

• Thin Layer Chromatography

• Thin Liquid Chromatography

In TLC substances are separated because of differences in_______. Tick all that apply.

• polarity

• molecular size

• concentration

• electronegativity

The distance travelled by the compound depends on how soluble it is in the chosen solvent.

• TRUE

• FALSE

The distance travelled by the compound depends on how well it adheres to the plate.

• TRUE

• FALSE

What is normally required to visualise the spots on a TLC chromatogram? Tick all that apply.

• X-rays

• Ultraviolet light

• Infrared light

• Developing agent

How to calculate Rf?

• Rf = distance travelled by sample / distance travelled by solvent

• Rf = distance travelled by solvent / distance travelled by sample

A compound always has the same Rf value under the same conditions:______. Tick all that apply.

• temperature

• solvent

• saturation levels

• solvent volume

When could a co-spot be used?

• to make a direct comparison on a TLC plate between the tested compound and the pure substance

• to improve the solubility of the tested compound in the solvent

• to increase the Rf value

• to decrease the Rf value

The presence of more than one spot on the TLC plate shows that____

• the Rf value is higher than it should be

• the solvent used was too dilute

• impurities are present

• too much solvent was used

TLC can be used to_____. Tick all that apply

• assess the purity of substances

• confirm the identity of a compound

• separate a mixture of immiscible liquids

• purify a substance