AP Bio Insta-Review Topic 6.8: BioTechnology

Which DNA tech separates fragments by size/charge?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

Why does the DNA move towards positive end?

• Deoxyribose is negatively charged 

• Deoxyribose is positively charged

• Phosphate is negatively charged 

• Phosphate is positively charged

Which fragments travel farther?

• Small

• Large

Tags

NGSS.MS-PS2-2

NGSS.MS-PS3-1

What cuts the DNA prior to running gel electrophoresis?

• DNA polymerase

• Helicase

• Ligase

• Restriction enzyme

Minimum restriction sites for ECO on plasmid to get this?

• 1

• 2

• 3

• 4

Which suspect was at crime scene?

• A

• B

• C

• D

Which DNA tech is responsible for multiple copies of a gene?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

Which DNA tech involves adding plasmid to prokaryote?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

Plate has non transformed bacteria & no antibiotics

• I

• II

• III

• IV

If plasmid also had the gene to synthesize insulin... Which plate has the highest % of insulin producing bacteria?

• I

• II

• III

• IV

Tags

NGSS.HS-LS1-1