Exercise 11. BUN

What is the term/name for the reaction in the old method?

• Diacetylmonoxime reaction

• Fearon’s reaction

• Nelson Somogyi

• Radical reaction

Composition of the protein precipitant used in the old method

• 0.3N Ba(OH)2

• Arsenic Acid

• 2-Oxoglutarate

• 5% ZnSO4

What are the 2 steps involved in the condensation taking place in the old method?

• Boiling H2O bath

• CHON precipitation

• Cooling under running tap H2O

• Reading against a reagent blank

TRUE or FALSE: DAM is a direct method because it measures ammonia instead of urea

• TRUE

• FALSE

TRUE or FALSE: Ammonia-containing anticoagulants are contraindicated in the old method

• TRUE

• FALSE

In the new method, what is the wavelength of choice?

• 480nm

• 530nm

• 340nm

• 515nm

Concentration of the Urea standard used in the new method

• 100g/dL

• 20mg/dL

• 50mg/dL

• 10mg/dL

What is the conversion factor for BUN?

The indicator enzyme involved in the new method

• Glucose-6-phosphate dehydrogenase

• Lactate dehydrogenase

• Urease

• Glutamate dehydrogenase

End product measured in new method

• Diazine (yellow)

• NADH

• Diacetyl

• NAD