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Sequencing

Authored by Paula Ruiz

Biology

1st - 3rd Grade

Used 28+ times

Sequencing
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7 questions

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1.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

What generation do real-time sequencers belong to?

1st generation

3rd generation

Next Generation Sequencing

2.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

Media Image

This image represents which high throughput sequencing method

Sanger sequencing

Nanopore

PacBio

Illumina

3.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

Media Image

·         What sequencing technology is represented in the image?

Illumina

454

PacBio

Nanopore

4.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

·         Imagine that you are in an expedition across the Arctic Ocean and that you need to find the bacterial diversity in a sample you just obtained. Which sequencing technology is more advisable to use and why?

Illumina, because it brings the most reliable results.

Nanopore, because minion is portable and easy to use outside the lab.

Nanopore, because it is the only technology that allows us to find bacterial diversity.

PacBio, because it is always better to use this technology.

5.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

·         The main advantage of third generation sequencing regarding next-generation sequencing is…

Less preparation and sequencing time.

More accuracy of the sequences.

Starting material is the single-molecule, it doesn´t require prior PCR.

Cheaper technologies.

6.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

·         What considerations must we keep in mind while loading our flowcell?

Avoid introducing air bubbles into the flow cell.

Close priming port and spot on port before starting the run.

Remove air from the flowcell before adding the priming mix.

All answers are correct.

7.

MULTIPLE CHOICE QUESTION

3 mins • 1 pt

·         What is the purpose of the 16S PCR performed in this protocol?

Amplification of a small region of 16S gene.

Amplification of the whole 16S gene, addition of the corresponding index and the first adaptors needed to sequence.

Amplification of all sample’s DNA.

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