Ex 12A Creatinine

What is the collective name of the CHON precipitants used in Ex 12A?

• Nelson Somogyi

• Jaffe

• Fearons

• Folin Wu

CHON ppts used

• 0.3N Ba(OH)2

• 2/3N H2SO4

• 5% ZnSO4

• 10% Na Tungstate

Temperature of the reaction

• 37C

• Room Temp

• Ref Temp

• 100C or boiling temp

Wavelength used

• 340nm

• 480nm

• 515nm

• 540nm

What was used in the assay to set the %Transmittance to 100 and absorbance to 0?

Effect of lipemic samples

• False increase

• False decrease

• No effect

Effect of hemolyzed samples

• False increase

• False decrease

• No effect

Composition of alkaline picrate in this method

• 1ml 10% NaOH

• 5 ml Saturated Picric Acid

• 5ml 2/3N H2SO4

• 1ml 10% Na Tungstate

What chemical can enhance/improve sensitivity and specificity of the Jaffe method?

Conversion factor for Creatinine

• 144.5

• 0.357

• 88.4

• 2.14