REINFORCEMENT 1 DB014 - Mutation & Recombinant DNA Technology

In sickle cell anaemia, glutamic acid is replaced with valine.

Identify the type of gene mutation that has occurred.

• Base deletion.

• Base insertion.

• Inversion.

•   Base substitution.

A _____________ is a nucleotide base substitution that results in new amino acid. 

• nonsense mutation.         

            

•   silent mutation. 

• missense mutation.        

• point mutation. 

Cri du chat is a chromosomal condition that results when a piece of number 5 chromosome is missing.  

What type of chromosomal mutation that has occurred?

• Deletion

                    

              

• Inversion

• Duplication

• Translocation

What type of gene mutation has occurred when UAU is changed to UCC?  

• Base substitution.  

           

   

• Base insertion.

• Inversion. 

• Base deletion.  

EcoR1 is an example of enzyme that recognizes and cuts DNA molecules at a specific recognition site. This is an example of a  

• DNA ligase

• DNA polymerase

• RNA polymerase

•   restriction enzyme

           

The mechanism where a recombinant DNA is introduced into host cells is called 

• transduction. 

•   conjugation.

• amplification. 

• transformation.

Which pair of enzymes are needed in gene technology?      

• RNA polymerase and DNA ligase

• DNA polymerase and DNA ligase

• DNA ligase and restriction enzyme

• RNA polymerase and restriction enzyme

A small and circular DNA that is used as a cloning vector is  

• Escherichia coli

           

•   plasmid

• target DNA

• bacteria