Biotechnology

An application of DNA technology to help environmental scientists would be 

• use PCR to analyze DNA at a crime scene

• create a tobacco plant that glows in the dark

• clone the gene for human growth hormone to treat dwarfism

• make transgenic bacteria that can be used to clean up oil spills more quickly than natural bacteria

Which of the following would be an example of gene therapy technology?

• cutting DNA into fragments with restriction enzymes

• development of nasal spray that contains copies of a normal gene that is defective in person with cystic fibrosis

• modifying E. coli to produce indigo dye for coloring denim blue jeans

• separation of DNA fragments using Gel Electrophoresis

Gel electrophoresis is a technique used to

• cut DNA into fragments of various sizes

• clone chromosomes of various species

• inject foreign DNA into animal and plant cells

• separate DNA fragments by charge and length

A small amount of DNA obtained from a mummy or from frozen remains of a human may be cloned. In order to clone small amounts of DNA, _____ needs to be used to generate larger quantities of the DNA.

• polymerase chain reaction techniques

• gel electrophoresis

• DNA Fingerprinting

• gene splicing

In 1974, Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacterium. The bacterium produced the protein coded for by the inserted frog gene. The bacterium containing functional frog DNA would be classified as a _____.

• clone

• DNA fingerprint

• plasmid

• transgenic organism

Who does the blood stain belong to?

• Sue

• Bob

• John

• Lisa

The process used to separate DNA segments of different lengths is

• gel electrophoresis

• PCR

• gene amplification

• all of these

Listed below are procedures involved in the production of a transgenic organism. From the choices provided, select the sequence that represents the proper order of events.

1. Recombinant DNA is transferred into a bacterial cell.

2. A specific gene is identified in a DNA sequence.

3. The DNA fragment is recombined into a vector.

4. The DNA fragment to be inserted is isolated.

• 2 3 1 4

• 1 2 3 4

• 4 1 2 3

• 2 4 3 1

The Human Genome Project has involved sequencing and mapping the human genome. The most important benefit of this information has been the diagnosis of genetic disorders. Once a genetic disorder is diagnosed, ______ can be used as a possible treatment.

• PCR

• cell cultures

• gene therapy

• DNA fingerprinting

Which of the following are applications of genetic engineering?

• all of these

• transgenic bacteria in agriculture

• transgenic bacteria in industry

• transgenic plants and animals