
Final Review
Quiz
•
Biology
•
9th - 12th Grade
•
Easy
Emily Scheib
Used 7+ times
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28 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the function of reverse transcriptase?
To synthesize RNA from DNA
To synthesize DNA from RNA
To synthesize proteins from genes
To break phosphodiester bonds
2.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
A bacteria has a mutation that causes the lacY gene to be defective, meaning that galactose permease is inactive. What effect(s) will this have on the bacteria?
Lactose will overaccumulate in the cell, so gene expression will be constitutive
Lactose will not be imported into the cell, so gene expression will be constitutive
Lactose will be imported into the cell, but it cannot be broken down
Lactose will not be imported into the cell, so transcription will not be induced.
3.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
Put the steps of transcription initiation (for bacteria) in the correct order:
RNAP opens the DNA helix, sigma binds to the promoter, sigma is released.
Sigma binds to the promoter, sigma is released, RNAP opens the DNA helix
Sigma binds to the promoter, RNAP opens the DNA helix, sigma is released
RNAP opens the DNA helix, sigma is released, sigma binds to the promoter
4.
MULTIPLE CHOICE QUESTION
45 sec • 1 pt
Does transcription begin downstream or upstream of the -35 box in prokaryotes?
Downstream
Upstream
Answer explanation
Sigma binds to the -10 and -35 box, so the proceeding transcription must occur downstream (to the right of the operon)
5.
MULTIPLE CHOICE QUESTION
1 min • 1 pt
As an undergraduate researcher in the lab, you are attempting to produce protein by utilizing bacterial E. Coli cells. In order to express your protein, a repressor on the lac operon must be removed. Your two options to induce expression are IPTG or lactose. IPTG is almost identical in structure to lactose, but it cannot be broken down by B-galactosidase. Which would be better to use?
IPTG because it cannot be broken down by B-galactosidase and its concentration will therefore remain constant
Lactose because IPTG cannot bind to the repressor on the lac operon
IPTG because it cannot be broken down by B-galactosidase and its concentration will therefore increase overtime
Lactose because it is most similar in structure to glucose, which provides energy for the cell
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
A scientist wants to genetically modify a mushroom to resist browning by using CRISPR. When he designs his experiment, however, he adds the wrong base pair to the single guide RNA (sgRNA). What will be the greatest issue with this design flaw?
The sgRNA will not be able to bind to the tracrRNA
The Cas9 enzyme will lose its endonuclease ability
The DNA cannot be repaired after cleaving
The Cas9 enzyme will not be able to locate the sequence of interest
7.
MULTIPLE CHOICE QUESTION
45 sec • 1 pt
Under what conditions is expression greatest in bacterial cells?
High lactose, low glucose, high cAMP
Low lactose, high glucose, high cAMP
High lactose, low glucose, low cAMP
Low lactose, high glucose, low cAMP
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