GFP Lab Pop Quiz

The process in which bacteria takes up exogenous DNA from the surrounding environment is known as

• transformation

• conjugation

• transduction

• recombination

Bacteria that are able to take up DNA from their environment are called

• competent

• vulnerable

• transformable

• recombined

The selectable marker on the plasmid used in this lab is a gene that codes for resistance to

• ampicillin

• vancomycin

• beta-lactamase

• penicillin

Green fluorescent protein (GFP) originates in

• jellyfish

• frogs

• C. elegans

• algae

In order to fluoresce, GFP must first be exposed to

• UV light

• CaCl2

• ampicillin

• heat

Which action will be done to encourage the bacterial cells to take up the plasmid from the environment?

• heat shock

• freezing

• addition of recovery broth

• plating on ampicillin

The method you will use to separate GFP from other cell proteins is called

• column chromatography

• SDS-PAGE

• electrophoresis

• centrifugation

Column chromatography will separate the cellular proteins according to

• size

• charge

• shape

• fluorescence

Adding SDS to the isolated GFP will give it which property?

• negative charge

• positive charge

• tight folding

• acidic

• basic

After performing electrophoresis, you will determine the molecular weight of GFP with the help of a(n)

• standard curve

• scale

• microscope

• fluorescence meter

• column chromatography