QUIZ: UMBI Ion S5 Prime

Who is she?

• Anna

• Elsa

• Olaf

• Rapunzel

Ion Torrent DNA Sequencing is based on which of the following detection technology?

• Dye based sequencing

• Fluorescent sequencing

• Semiconductor sequencing

• DNA molecule Sequencing

What is the correct sequencing workflow that applies in the Ion Torrent Sequencing technology?

• Template preparation, Sequence run, Library preparation, Data analysis

• Data analysis, Library preparation, Template preparation, Sequence run

• Sequence run, Data analysis, Template preparation, Library preparation

• Library preparation, Template preparation, Sequence run, Data analysis

How many DNA and RNA primers pools are provided in the Oncomine Childhood Cancer Research Assay?

• 1 and 1

• 1 and 2

• 2 and 1

• 2 and 2

Different (a)   must be ligated to each library when sequencing multiple libraries on a single run.

Which of the following library quantification methods are recommended for OCCRA workflow?

• qPCR quantification

• Qubit

• Bioanalyzer

• Nanodrop

What is Kermit?

• Toad

• Frog

• Pig

• Bear

The purified and pooled sample library should be loading into which position in Ion Chef?

• 5

• 9

• 1 and 2

• 8

Which of the following statement about the clonal amplification is incorrect?

• Monoclonal and polyclonal products are both yielded during the clonal amplification

• Clonal amplification is also referring to the Emulsion PCR

• The more library molecules present in an oil droplet, the better the amplification results

• Library, nucleotide, Taq polymerase, ISP, buffer and oil are the components needed for the clonal amplification.

ISP without the bound of DNA will be removed during the (a)   step.