DNA Profiling

So which rabbit was the father?

• Husband

• Suspect

• Standard

• Both Husband and Suspect

What is the best use for the Standard in DNA profiling?

• Gives a step ladder of sizes to compare to

• The Standard is a negative control

• The Standard fragments all contain CAT sequences

• The Standard charges the gel so that DNA fragment move

What percentage of the child DNA came from the mother?

• 100%

• 75%

• 50%

• 25%

Why was it necessary to use a restriction enzyme on the DNA before the gel electrophoresis?

• The enzyme cuts pores in the gel making movement possible

• Impossible to compare DNA if all samples are one long fragment

• The enzyme acts to negatively charge the DNA

• The enzyme eliminates contaminating protein

What property did the gel electrophoresis use to separate the DNA fragments?

• Smaller fragments are more positively charged

• Smaller fragments move faster

• DNA fragments with more A-T bases move faster

• DNA fragments that are more linear than spherical move faster

Why was it necessary to use a GTA fluorescent probe?

• DNA is only visible with a fluorescent marker

• Comparison of Rabbit DNA fragments with the Standard told us the fragment size

• Some DNA fragments were the same size but had different bases

• All the Rabbit samples had the same DNA patterns

Why did all the rabbits have so many fragments in common?

• All the rabbits in this lab were closely related

• All rabbits have 99% of DNA in common

• Only a small portion of the chromosomal DNA was examined

• The GTA probe only examined related fragments