bio-200 feb 8

we purify the phage lysate to try and get rid of

• bacterial dna

• bacterial proteins

• bacterial lipids

• all of these

the bacterial dna will be destroyed by

• nutreases

• nucleases

• genzymes

• proteinases

phage dna will not be degraded because

• it has an anti-enzyme

• it is surrounded by a membrane

• there is a high salt concentration

• it has a capsid

guanidine hydrochloride

• inactivates nucleases

• breaks down the capsid

• disrupts membranes

• destroys nucleotides

we will be using the _____ dna clean up kit

• concentrator

• warlock

• wizard

• purity

we wash our columns with

• 100% ethanol

• 70% ethanol

• 60% isopropanol

• 80% isopropanol