Gene and cDNA libraries

Types of gene libraries (pick more than one answers)

• cDNA

• mRNA

• Genomic

• All of the following

What is a cDNA?

• collection of total genomic DNA

• non-coding RNA molecule (containing about 22 nucleotides) found in plants,

• complementary DNA molecules synthesized from mRNA

• small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules

A gene library is a collection of restriction enzymes where each contain a portion of the genetic material of interest .

• True

• False

Where do we insert vectors?

• bacterial host cells

• restriction enzymes

• culture media

• a and b only

Below are correct statements regarding a probe EXCEPT

• A probe is used to search for its complementary sequence in a sample genome.

• The probe is labeled with a radioactive or chemical tag that allows its binding to be visualized

• A probe is a single-stranded sequence of DNA or RNA

• A probe is a double-stranded sequence of DNA or RNA

A cDNA library is used when we want to study

• the transcriptome.

• the genome.

The diagram showed the steps in creating a genomic library. Step 1 starts with genomic DNA extraction. Step 2 that followed would be

• Transformation into appropriate vecors

• Partial digestion with restriction enzymes or physical shearing

The diagram showed the steps in genomic library creation. The last step is screening for positive clones. Screening includes all the methods below EXCEPT

• colony hybridization screening with a probe

• screening on a substrate specific medium

• screening with polymerase chain reaction (PCR)

• screening using Western blotting

A genomic library can be built in a

• phage library form

• plasmid library form

• YACs library form

• All of above

Which ADDITIONAL feature is essential for expression vectors?

• multiple cloning site

• promoter

• tag sequences

• selection marker