BIOL1012 Practical 1

Gel electrophoresis separates molecular products based on:

• Shape

• Size

• Charge

• Evolutionary similarity

When traveling through an agarose gel, larger molecular products will migrate _________ smaller molecular products

• Faster than

• Slower than

• At the same rate

Buffer is used instead of water when making and running gels. Which of the following is NOT true for buffer?

• Buffer enhances the transmission of electric currents in water

• Buffer is more homogeneous than water

• Buffer neutralizes the charge on molecular products prior to electrophoresis

• Buffer neutralizes the charge on molecular products prior to electrophoresis

The size of the molecular products is determined by

• The intensity of the band

• The percentage of agarose gel

• Comparison with a molecular weight “ladder”

• Being familiar with your gel and “eyeballing” it

In a laboratory, the following should not be worn

• loose clothing

• dangling jewelry

• sandals.

• All options here

The following footwear is best in the laboratory

• sandals

• open-toed shoes

• closed-toed shoes

• shoes appropriate for the weather

Which of the following lens objective would you use to obseve pathological samples in great details ?

• X4

• X10

• X40

How is the image under the microscope compared to image through normal eyes ?

• Backward and upright

• Backward and upside down

• Upside down only

• No differences

How does centrifuge separate a heterogenous mixture?

• Spinning the fluid at high speed

• Spinning the fluid at low speed

• Standing still under low pressure

• Standing still under low temperature

Which of the following is NOT allowed/recommended when operating the centrifuge?

• All tubes/containers are properly balanced in the rotor

• Any tubes/containers can be used in the centrifuge

• The centrifuge lid should be closed during operation

• All tubes/containers should not be overfilled