ANTIGLOBULIN TEST

Contain antibody to human IgG and to the C3d component of human complement. Can facilitate agglutination when RBCs have been sensitized with IgG or C3d or both

• POLYSPECIFIC AHG REAGENTS
• MONOSPECIFIC AHG REAGENTS

Contain only one antibody specificity: either anti-IgG or antibody to specific components of complement, such as C3b or C3d

• POLYSPECIFIC AHG REAGENTS
• MONOSPECIFIC AHG REAGENTS

Contain antibodies specific for the Fc fragment of the gamma heavy chain of the IgG molecule

• Anti-IgG reagents
• Anticomplement reagents

Reactive against only the designated complement components and contain no activity against human immunoglobulins

• Anti-IgG reagents
• Anticomplement reagents

Usually prepared in rabbits. Modern production commences with the purification of the immunogen from a large pool of normal sera. Separate blends of the anti-IgG and anticomplement antibodies are made, and each pool is then absorbed with A1, B, and O cells to remove heterospecific antibodies

• POLYCLONAL AHG
• MONOCLONAL AHG

All antibody molecules produced by a clone of hybridoma cells are identical in terms of antibody structure and antigen specificity. All antibodies produced by a clone of cells recognize a single epitope present on an antigen. There is no need for absorption to remove heterospecific antibodies

• POLYCLONAL AHG
• MONOCLONAL AHG

MONOCLONAL AHG PRODUCTION are devised by

• Kohler and Milstein
• Landsteiner and Weiner

Detects in vivo sensitization of RBCs with IgG or complement components.

• DIRECT ANTIGLOBULIN TEST (DAT)
• INDIRECT ANTIGLOBULIN TEST (IAT)

Performed to determine in vitro sensitization of RBCs

• DIRECT ANTIGLOBULIN TEST (DAT)
• INDIRECT ANTIGLOBULIN TEST (IAT)

Maternal antibody coating fetal RBCs

• Hemolytic disease of the fetus and newborn (HDFN)
• Hemolytic transfusion reaction (HTR)
• Autoimmune and drug-induced hemolytic anemia (AIHA)