Transformation Lab Questions

In bacterial transformation, CaCl2 is added to

• shield the positive charge on the DNA

• change the charge of DNA from positive to negative

• shield the negative charge on the DNA

• stop the DNA from replicating

What is genetic transformation?

• A prokaryotic cell turning into a eukaryotic cell

• Foreign DNA being inserted into a plasmid

• A host organism taking in foreign DNA

• A gene being mutated

What is the purpose of the Luria broth (LB) when performing genetic transformation?

• Provides resistance to antibiotics

• Provides nutrients needed for bacteria growth

• Stabilizes the plasmid being given to the host organism

• Sterilizes the instruments

A "-amp^R" cell

• has the amp^R gene in it's plasmid and is resistant to ampicillin

• does not have the amp^R gene in it's plasmid and is resistant to ampicillin

• has the amp^R gene in it's plasmid and is not resistant to ampicillin

• does not have the amp^R gene in it's plasmid and is not resistant to ampicillin

Tags

NGSS.HS-LS1-1

Which one of these agar plates contains bacteria most like the original bacterial culture

• +pGLO LB/amp

• +pGLO LB/amp/ara

• -pGLO LB/amp

• -pGLO LB

Which one of these agar plates would there be no bacteria growth?

• +pGLO LB/amp

• +pGLO LB/amp/ara

• -pGLO LB/amp

• -pGLO LB

The plasmids scientists use in a lab are MOST COMMONLY found in

• plants

• animals

• archaea

• bacteria

Arabinose is used in the plates to

• isolate and select for only the bacteria that have been successfully transformed

• give bacteria energy to grow

• act as an inducer to turn on the expression of the gene of interest

• give the bacteria a new trait (such as fluorescence or insulin production)

What is the vehicle for getting the GFP gene into the e.coli?

• the plasmid

• chromosomal DNA from the jellyfish

• heat shock

• transcription

if we say that the e.coli is TRANSFORMED, what does this mean?

• it is no longer a prokaryote

• it has more jellyfish DNA > e.coli DNA

• it has taken up the pglo plasmid

• it has taken up the pglo plasmid and expressed the genes (think transcription and translation)