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Transformation Lab Questions

Authored by Kristin Umile

Science

12th Grade

NGSS covered

Used 2+ times

Transformation Lab Questions
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15 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

In bacterial transformation, CaCl2 is added to

shield the positive charge on the DNA

change the charge of DNA from positive to negative

shield the negative charge on the DNA

stop the DNA from replicating

2.

MULTIPLE CHOICE QUESTION

30 sec • 5 pts

What is genetic transformation?

A prokaryotic cell turning into a eukaryotic cell

Foreign DNA being inserted into a plasmid

A host organism taking in foreign DNA

A gene being mutated

3.

MULTIPLE CHOICE QUESTION

30 sec • 5 pts

What is the purpose of the Luria broth (LB) when performing genetic transformation?

Provides resistance to antibiotics

Provides nutrients needed for bacteria growth

Stabilizes the plasmid being given to the host organism

Sterilizes the instruments

4.

MULTIPLE CHOICE QUESTION

30 sec • 5 pts

A "-ampR" cell

has the ampR gene in it's plasmid and is resistant to ampicillin

does not have the ampR gene in it's plasmid and is resistant to ampicillin

has the ampR gene in it's plasmid and is not resistant to ampicillin

does not have the ampR gene in it's plasmid and is not resistant to ampicillin

Tags

NGSS.HS-LS1-1

5.

MULTIPLE CHOICE QUESTION

30 sec • 5 pts

Media Image

Which one of these agar plates contains bacteria most like the original bacterial culture

+pGLO LB/amp

+pGLO LB/amp/ara

-pGLO LB/amp

-pGLO LB

6.

MULTIPLE CHOICE QUESTION

30 sec • 5 pts

Media Image

Which one of these agar plates would there be no bacteria growth?

+pGLO LB/amp

+pGLO LB/amp/ara

-pGLO LB/amp

-pGLO LB

7.

MULTIPLE CHOICE QUESTION

30 sec • 5 pts

The plasmids scientists use in a lab are MOST COMMONLY found in

plants

animals

archaea

bacteria

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