GRAM (+) COCCI, CATALASE (+) ... CONTINUATION

the initial presumptive identification method for all gram positive cocci

• Gram staining

• Catalase test

• Modified oxidase test

• Disk test

medium to large smooth, opaque, creamy yellow pigmented colonies

• 5% Sheep Blood Agar

• Phenylethyl Alcohol (PEA) or Columbia colistin nalidixic acid (CNA)

• Mannitol Salt Agar

• Leoffler's Medium

used to eliminate contamination by gram-negative organisms in heavily contaminated specimens

• 5% Sheep Blood Agar

• Phenylethyl Alcohol (PEA) or Columbia colistin nalidixic acid (CNA)

• Mannitol Salt Agar

• Leoffler's Medium

contains high concentration of SALT(10%), MANNITOL and PHENOL RED

• 5% Sheep Blood Agar

• Phenylethyl Alcohol (PEA) or Columbia colistin nalidixic acid (CNA)

• Mannitol Salt Agar

• Leoffler's Medium

S. aureus FERMENTS MANNITOL and produces a YELLOW HALO on this media

• TRUE

• FALSE

Enhances pigmentation of Stahylococcus

• 5% Sheep Blood Agar

• Phenylethyl Alcohol (PEA) or Columbia colistin nalidixic acid (CNA)

• Mannitol Salt Agar

• Leoffler's Medium

golden yellow colonies

• S. aureus

• S. citreus

• S. albus

lemon yellow colonies

• S. aureus

• S. citreus

• S. albus

porcelain white colonies

• S. aureus

• S. citreus

• S. albus

Staphylococcus (+)

Micrococci (+)

Streptococci (-)

• Catalase test

• Modified oxidase test

• Disk test

• Coagulase