GENERAL PRINCIPLES IN MICROBIOLOGY (PART 2)

Urine preservation

• Boric acid
• Polyvinyl alcohol
• Formalin
• Stuart’s medium

Ova, parasite, trophozoites, and cyst

• Boric acid
• Polyvinyl alcohol
• Formalin
• Stuart’s medium

Transport media that contains swab and is activated by crushing an ampule

• Boric acid
• Polyvinyl alcohol
• Formalin
• Stuart’s medium

Modified Stuart’s medium

• Amie’s medium
• Cary-Blair medium

For stool specimen (enteric pathogens)

• Amie’s medium
• Cary-Blair medium

Anticoagulant for blood culture, bone marrow, and synovial fluid

• Sodium polyaenthol sulfonate
• Sodium thioglycolate
• JEMBEC system

Maintain viability for up to 72 hours

• Sodium polyaenthol sulfonate
• Sodium thioglycolate
• JEMBEC system

Serves as N. Gonorrhea transport medium which contains selective agar and CO2 generating tablet.

• Sodium polyaenthol sulfonate
• Sodium thioglycolate
• JEMBEC system

Areas with blood or mucus must be located and sampled for culture and direct examination. Note the appearance of specimen being received whether it is blood, cloudy, clotted, etc.

• Gross Examination of Specimen
• Direct microscopic examination

Serves as quality assessment of specimen, for example the assessment of sputum using Bartlett’s criteria. Early indication of infectious agent (e.g., presence of WBC and gram [+] diplococci indicative S. pneumoniae

• Gross Examination of Specimen
• Direct microscopic examination