Basic Immunologic response

* use of labeled monoclonal antibodies against specific surface antigens

* light scattering is measured as cells flow through a laser beam

• Cell Flow Cytometry

• Rosette Technique

• Direct or Indirect Immunofluorescence

• Surface Immunoglobulins

• Density gradient centrifugation with Ficoll-Hypaque

* old method

* T lymphocytes form rosettes with sheep's RBC (CD2- receptor for sheep's RBC)

• Cell Flow Cytometry

• Rosette Technique

• Direct or Indirect Immunofluorescence

• Surface Immunoglobulins

• Density gradient centrifugation with Ficoll-Hypaque

* use of labeled monoclonal antibodies against specific surface antigens

* slides are read with a fluorescent microscope

• Cell Flow Cytometry

• Rosette Technique

• Direct or Indirect Immunofluorescence

• Surface Immunoglobulins

• Density gradient centrifugation with Ficoll-Hypaque

* immunoenzymatic method

* demonstrate immunoglobulins on B cells (Y-shaped molecule)

• Cell Flow Cytometry

• Rosette Technique

• Direct or Indirect Immunofluorescence

• Surface Immunoglobulins

• Density gradient centrifugation with Ficoll-Hypaque

* to separate B to T

* use nylon wool as a strainer to filter the lymphocytes

• Cell Flow Cytometry

• Rosette Technique

• Direct or Indirect Immunofluorescence

• Surface Immunoglobulins

• Density gradient centrifugation with Ficoll-Hypaque

*monocytes, macrophages, DCs

* inflammation

* fever initiation of the acute phase response

• Interleukin-1β

• Tumor necrosis factor - alpha

• Interleukin - 6

• Transforming growth factor-beta

• Interferon - alpha

  Interferon - beta

monocytes

macrophages

neutrophils

NK cells

activated T cells

• Interleukin-1β

• Tumor necrosis factor - alpha

• Interleukin - 6

• Transforming growth factor-beta

• Interferon - alpha

  Interferon - beta

monocytes macrophages

endothelial cells

Th2 cells

• Interleukin-1β

• Tumor necrosis factor - alpha

• Interleukin - 6

• Transforming growth factor-beta

• Interferon - alpha

  Interferon - beta

T cells

macrophages

other cell types

• Interleukin-1β

• Tumor necrosis factor - alpha

• Interleukin - 6

• Transforming growth factor-beta

• Interferon - alpha

  Interferon - beta

macrophages

DCs

virally infected cells

• Interleukin-1β

• Tumor necrosis factor - alpha

• Interleukin - 6

• Transforming growth factor-beta

• Interferon - alpha

  Interferon - beta